Diacylglycerol kinase-? restores cardiac dysfunction under chronic pressure overload: a new specific regulator of Gaq signaling cascade

摘要

Gaq protein-coupled receptor (GPCR) signaling pathway, which includes diacylglycerol (DAG) and protein kinase C (PKC), plays a critical role in cardiac hypertrophy. DAG kinase (DGK) catalyzes DAG phosphor-ylation and controls cellular DAG levels, thus acting as a regulator of GPCR signaling. It has been reported that DGKe acts specifically on DAG produced by inositol cycling. In this study, we examined whether DGKe prevents cardiac hypertrophy and progression to heart failure under chronic pressure overload. We generated transgenic mice with cardiac-specific overexpression of DGKe (DGKe-TG) using an a-myosin heavy chain promoter.