Ischemia-induced activation of AMPK does not increase glucose uptake in glycogen-replete isolated working rat hearts

摘要

Several studies have investigated ischemia-induced alterations in glucose uptake, but these have yielded conflicting conclusions. More than three decades ago, Neely and colleagues (35, 37) showed that ischemia reduces glucose uptake and glycolysis. However, those results contrast with positron emission tomography studies that show an increase in the uptake of 18F-deoxyglucose in relation to coronary flow in patients with coronary artery disease (10, 32). Similar observations in vivo (50) and ex vivo (40, 45) were attributed to a translocation of GLUT4 to the cell surface, due to ischemia-induced activation of adenosine 5'-monophosphate-activated protein kinase (AMPK) (39).