Epithelial Na~+ channel activation and processing in mice lacking SGK1

摘要

Generation of SGK1 knockout mice. All experimental protocols were approved by the Institutional Animal Use and Care Committees Of Dartmouth Medical School and the Weill Medical College of Cornell University, and all procedures using experimental animals adhered to the American Physiological Society's "Guiding Principles in the Care and Use of Animals." Floxed SGK1 mice were generated by the Transgenic and Knock-out Mouse Core Facility at Dartmouth.Positive ES clones were microinjected into C57BL6-derived blas-tocysts and implanted into pseudopregnant foster females. Progeny were identified by PCR analysis of tail DNA (PCR conditions are available upon request), and Southern blot analysis was used to verify integration of the transgene. Figure 1 shows a Southern blot of DNA from wild-type mice (+/+) and from mice heterozygous for the floxed SGK1 allele (+/-).