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The application of molecular marker technology in the classification and breeding of Chrysanthemum.

机译:分子标记技术在菊花的分类育种中的应用。

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Chrysanthemum ( Chrysanthemum * morifolium Ramat.) is a very important commodity flower, and is also widely used in the landscape. But the classification of chrysanthemum cultivars has been controversial. Chrysanthemum classification research has continued from the traditional morphological classification to application of using molecular markers, as auxiliary tool. The results of molecular marker clustering analysis of wild and cultivated chrysanthemum and relatives were not fully congruent with the morphological classification. In terms of origin, the study of RAPD showed that Chrysanthemum vestitum was the most important species in the origin process of cultivated chrysanthemum. Research into the genetic relationships of chrysanthemum taxa using molecular marker methods such as AFLP, SSR, ISSR, and RAPD and the application of these to the classification and breeding of chrysanthemum were discussed. Among these methods, ISSR was seen as the best, and it was combined with the high polymorphism of AFLP, SSR and the commonality of RAPD. Sometimes, the same molecular marker method yielded different results in the different studies. The differences and similarities between classification methods in China and elsewhere were discussed in terms of molecular marker technology and morphology. With the combination of molecular marker technology and traditional morphological approaches, breeding success will be improved. It will clarify the cross-compatibility among chrysanthemum cultivars and expand the range of breeding resources for genetic improvement. The breeding period will be shortened with the directional transfer of favorable genes and the accumulation of target genes. At the same time, this research will provide an accurate reference library for the protection of chrysanthemum germplasm resources and the innovation of taxonomy. CT VI International Symposium on the Taxonomy of Cultivated Plants, Beijing, China.
机译:菊花(Chrysanthemum * morifolium Ramat。)是一种非常重要的商品花,在景观中也被广泛使用。但是,菊花品种的分类一直存在争议。菊花的分类研究已从传统的形态学分类继续发展到使用分子标记作为辅助工具的应用。野生和栽培菊花及其近缘种的分子标记聚类分析结果与形态分类不完全一致。在起源方面,RAPD的研究表明,菊花菊是栽培菊花起源过程中最重要的物种。讨论了使用AFLP,SSR,ISSR和RAPD等分子标记方法研究菊花类群的遗传关系,并将其应用于菊花的分类育种。在这些方法中,ISSR被认为是最好的,并与AFLP,SSR的高多态性和RAPD的通用性相结合。有时,相同的分子标记方法在不同的研究中会产生不同的结果。从分子标记技术和形态学的角度探讨了中国与世界其他地区分类方法的异同。结合分子标记技术和传统形态学方法,将提高育种成功率。它将阐明菊花品种之间的交叉兼容性,并扩大遗传改良的育种资源范围。通过有利基因的定向转移和靶基因的积累,可以缩短繁殖期。同时,该研究将为菊花的种质资源保护和分类学创新提供准确的参考库。第六届国际CT栽培植物分类学研讨会,中国北京。

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