Advances in the in vitro culture and micropropagation of tree peonies during the past half century.

摘要

Tree peonies native to China are prized for their large and gorgeous flowers. However, difficulties of propagation and slow plant growth have long been an obstacle for extending and using this plant. Since first reported in 1965, tissue culture has interested many researchers to develop novel techniques for the mass propagation and shortening of the breeding cycle of tree peonies. This article will review advances made on various aspects of in vitro culture and micropropagation of the tree peonies during the past half century. Though micropropagation has shown spectacular development in recent years, its commercial use is believed to be still limited due to problems like excessive browning, lower multiplication rate and difficulty of rooting, etc. The plantlets from in vitro embryo and somatic embryogenesis were achieved, but some investigations are still needed on more commercial cultivars. Moreover, on a commercial scale for the production of tree peonies, protocols providing regeneration from various vegetative parts of the plants are needed. Given the information from published studies, we suggest further studies should develop at the base of genotypes in addition to a survey of the physiological state of explants to accurately determine the most appropriate culture conditions.