Structural characteristics of SLF/SFB -genes in apple cultivars with similar S -RNase sequences.

摘要

We have isolated SLF/SFB gene candidates in some apple cultivars. Pollen cDNA and genomic clones obtained from S₁, S₂₀, S₂₄, S₃₃ and S₃₄ haplotypes were grouped based on phylogenetic analysis. It was suggested that a haplotype-specific new gene, rather than SLFB1, was present in S₁. The gene was expressed in pollen, and its V1 to V4 regions, which seemed to be important for allele discrimination, and was different from those in the other haplotypes. In contrast, only a few amino acid differences within the V1 region were observed between SLFB1 and other haplotypes.