rnl Method for genetic grouping of cultivars in Pyropia yezoensis by using the structural variation of mitochondrial rnl gene

摘要

The present invention relates to a method for genetically and easily grouping varieties of radiolabelled plants utilizing the structural mutation of the mitochondrial rnl gene, comprising the steps of (A) using a genomic DNA isolated from radiolabeled rice as a template, A pair of primer sets consisting of a primer matching a single exon e (n) sequence of the mitochondrial large nucleotide ribosomal RNA ( rnl ) gene of Kim and a primer matching a complementary sequence of exon e (n + 1) Performing PCR for the three introns i1 to i3 of the mitochondrial rnl gene of the radiolabeled or related species; (B) confirming the amplification of each of the introns i1 to i3 in the amplification product; And (C) dividing the radiolabelled Kimchi varieties shown in the above table into at least five groups according to amplification of each of i1 to i3. According to the present invention, it is possible to group and group the varieties of radiolabeled rice varieties, thereby preserving and managing varietal varieties, selecting the closest control varieties and reference varieties to breed varieties, Seed dispute resolution, etc., it can be actively used for scientific breeding, protection of intellectual property rights and establishing a seed distribution system.