Genetic diversity revealed by fluorescent-AFLP markers for apricot germplasms in Shandong, China.

摘要

Apricot is originated in China. Shandong is a major province in apricot production in china and has abundant apricot genetic resources. During the long term of evolution and selection, a lot of local apricot cultivars such as Badanshuixing in Tai'an, Honghebao in Jinan, Guanyelian and Yangjiyuan in Qingdao were formed. The genetic diversity of 26 apricot cultivars in Shandong province was analyzed by the fluorescent-AFLP marker techniques. The results showed that the average number of polymorphic loci amplified by 8 pairs of fluorescent-AFLP primers was 103.25. The mean percentage of polymorphic loci (P) was 87.37%. All average Nei's gene diversity index (H) and average Shannon's information index (I) was 0.1935 and 0.3100 respectively, which indicated that genetic diversity of Shandong apricot was abundant. Apricot cultivars in Shandong were divided into 4 groups at 58.58% level of similarity coefficient. Shandong apricot local cultivars distributed in all four groups, in which 'Yubadan' and 'Yangjiyuan' cultivars were farther with other Shandong apricot local cultivars and American apricot in genetic distances. So 'Yubadan' and 'Yangjiyuan' were important for broadening genetic diversity of Shandong apricots, breeding new apricot cultivars and creating new germplasm.