Transmission characteristics of Pineapple mealybug wilt associated virus-2 by the grey pineapple mealybugs Dysmicoccus neobrevipes in Hawaii.

摘要

Pineapple mealybug wilt associated virus-2 (PMWaV-2) is a mealybug transmitted ampelovirus associated with mealybug wilt disease of pineapple (MWP) in Hawaii. The characteristics of PMWaV-2 acquisition and persistence of grey pineapple mealybugs (GPM), Dysmicoccus neobrevipes Beardsley, were measured. Some GPM were able to acquire the virus within 36 hours. Transmission efficiency of GPM in groups of 25 was 100% following a 72-hour acquisition access period (AAP). GPM remained viruliferous for up to three days after a 3-day AAP when serially transferred to virus-free pineapple plants at 24-hour intervals over a 7-day period. Access to a kabocha squash, a non-host of the virus, reduced the transmission efficiency and persistence of the virus. These data show that PMWaV-2 is transmitted by the grey pineapple mealybug in a semi-persistent manner. A potential management strategy based on these findings is discussed.