Changes of secondary metabolites content and phenolics metabolism-associated enzymes in phylloxera infested nodosities.

摘要

Secondary metabolites and their regulative enzyme in primary roots infested by phylloxera were tested. The results showed that secondary metabolites and their enzyme in primary roots had increased after inoculation. Also, the accumulation of total phenolics and lignin, the increased activity of phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO) and super oxide dismutase (SOD) were highly correlative with grape phylloxera resistance. The interrelating coefficients were 0.987, 0.841, 0.965, 0.909 and 0.782, respectively., On the 20th day after the phylloxera inoculation, the ratios of increased total phenolics content in '140 Ruggeri' were 33.68, 43.01 and 23.49% respectively higher than that of 'Tyoho', 'Tamina' and 'Crimson', and the increased lignin contents were 89.60, 43.64 and 27.08% higher than that of 'Tyoho', 'Tamina' and 'Crimson' as well. The increased activity of PAL, PPO and SOD were higher than other three cultivars.