Towards Sharka containment, the 'SharCo' Project: genetic approach.

摘要

Sharka disease, caused by Plum Pox Virus (PPV), produces important economic losses in Apricot (P. armeniaca L.). PPV natural resistance has been only found in some North American apricot cultivars and efforts are being made to introduce this character on Mediterranean germplasm. Recent studies on segregation of different intraspecific apricot crosses suggest that PPV resistance is controlled by at least one major dominant locus on the upper part of linkage group 1 (LG1). Using microsatellites already mapped in different Prunus spp. we have built a high density genetic map for an interval of 13 cM comprising this region in apricot. In addition, overgo probes designed from SSR clone sequences have been used to screen an apricot Bacterial Artificial Chromosome (BAC) library. Positive clones identified are being used to construct a BAC contig spanning the apricot genomic region containing the PPV resistance locus using the peach physical map developed by Zhebentyayeva et al. (2008) as reference.