Identification of S -alleles in several apple cultivars.

摘要

Two Polish apple cultivars 'Ligol' and 'Odra' and two Polish apple clones 'U633' and 'U1065' were utilized for studies of self-incompatibility. PCR amplification using FTC143 and FTC144 S-allele specific primers for S₇ failed to differentiate between the S₇ and S_z alleles. Based on available sequence data, a new set of primers upstream and downstream of the hypervariable region of apple S-RNases was designed. Using the RT-PCR method, cDNA was amplified on RNA isolated from styles of selected plants. PCR products were cloned and sequenced. In cultivar 'Odra' and clones 'U633' and 'U1065', a new S-allele, showing 97% of identity with Sauc19 derived from Sorbus aucuparia (EF494758) and denoted as a S-kb, was identified. The S_z allele was associated with cultivar 'Odra', and S₇ with 'U1065'. In 'Ligol', the agronomically most important cultivar in Poland, S₂ and S₉ are present.