Interaction of a single-stranded DNA-binding protein g5p with DNA oligonucleotides immobilised on a gold surface

摘要

We report surface plasmon resonance (spr) and confocal fluorescence results concerning the interaction of a gene-5-protein (g5p) with single-stranded DNA oligonucleotides (dT _(25)) tethered to a gold surface. The spr data show that a highly stable g5p-ssDNA complex is readily formed on a gold surface with k _(off) = 1.7 × 10 ~(-3) s ~(-1). The extent of the complexion indicated involvement of the cooperative protein-protein interactions within the binding to DNA. In the experiments where dT _(25) coexist with g5p in the solution, the fluorescence data show that g5p also mediates the binding between the non-complementary oligonucleotides in the solution and those immobilised on the surface.