G-CSF-induced aneuploidy does not affect CD34+ cells and does not require cell division.

摘要

Lymphocytes from granulocyte colony-stimulating factor (G-CSF)-mobilized donors display epigenetic and genetic alterations similar to those observed in leukemia patients. To further evaluate the scope and duration of G-CSF-induced genetic alterations, 24 healthy donors were followed for 1 year after peripheral blood stem cell mobilization by fluorescence in situ hybridization (FISH) analysis (Figure 1A). The chromosomes 8 and 17 aneuploidy increased an average of 4.5- and 4.3-fold, respectively, at the time of G-CSF mobilization and subsequently decreased, but remained significantly higher than baseline at 3 and 6 months after mobilization (Figure 1A). The frequency of cells with monosomy was higher, and returned to baseline more gradually than that of cells with trisomy or multisomy (data not shown).