首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Microbial production of 2,3 butanediol from seaweed hydrolysate using metabolically engineered Escherichia coli
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Microbial production of 2,3 butanediol from seaweed hydrolysate using metabolically engineered Escherichia coli

机译:使用代谢工程化大肠杆菌从海藻水解产物中微生物生产2,3丁二醇

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摘要

A variety of biofuel and biorefinery products have been produced from engineered Escherichia coli till date. Most of these products had been derived from simple sugars in its pure form, rather than deriving it from alternative, renewable and carbon neutral sources, such as marine alga biomass. Engineering E. coli to use algal hydrolysate can make these an attractive carbon source for the industrial production of value added fuels and chemicals. This work reports the engineering of E. coli by a combination of gene deletion and synthetic pathway incorporation, for the efficient utilization of algal hydrolysate to produce BA (2,3 butanediol. +. acetoin) under microaerobic condition. Engineered strain produced ~19. g/L of total BA from algal hydrolysate in defined M9 salt media at a yield of 0.43. g/g.
机译:迄今为止,工程化大肠杆菌已生产出多种生物燃料和生物精炼产品。这些产品大多数来自纯糖形式的简单糖,而不是来自替代,可再生和碳中和的资源,例如海藻生物质。对使用藻类水解产物的工程大肠杆菌进行改造,可以使其成为工业化生产增值燃料和化学品的诱人碳源。这项工作报告了通过基因缺失和合成途径掺入的组合对大肠杆菌进行工程改造,以在微需氧条件下有效利用藻类水解产物生产BA(2,3丁二醇+乙酰)。工程菌株产生了〜19。在定义的M9盐介质中,藻类水解产物产生的总BA量为g / L(0.43)。克/克

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