Engineering bacteria for bioremediation of persistent organochlorine pesticide lindane (γ-hexachlorocyclohexane)

摘要

Strategies were designed for bioremediation of the highly persistent toxic pesticide γ-hexachlorocyclohexane (γ-HCH) or lindane from the environment. Lindane caused the loss of stress-protective chaperone GroEL, and inhibited photosynthesis, respiration and nitrogen-fixation in Anabaena, resulting in growth arrest. To alleviate lindane toxicity, the linA2 gene, encoding HCH dehydrochlorinase from Sphingomonas paucimobilis B90, was knocked-in at an innocuous locus in Anabaena genome and over-expressed from an eco-friendly light-inducible P. _(psbA1) promoter. The recombinant Anabaena degraded 98% of 10. ppm lindane within 6-10. days. A LinA2 overexpressing Escherichia coli strain could degrade 10. ppm of all the isomers of lindane within 1. h and displayed a visual degradation zone on a newly designed histochemical plate containing 50. mg lindane within 12. h. The study demonstrates (a) bioremediation of traces of lindane prevalent in paddy fields, using bioengineered photoautotrophic Anabaena, and, (b) biodegradation of huge stockpiles of lindane, by employing recombinant live/dead E. coli.