Localized Surface Plasmon Resonance for Bioprocess Development, Monitoring, and Validation

摘要

Academic laboratories have embraced localized surface plasmon resonance (LSPR) as the "new wave" of label-free technology (1). This technique is based on the ability of colloidal metal nanoparticles or nanostructured metallic films to absorb light ina narrow wavelength range. Metal nanostructures "sense" changes occurring at their surfaces by shifting the frequency of the light they absorb or reflect. As a consequence, a basic LSPR system requires only optical fibers, a source of white light, and adetector (1,2). The simplicity of LSPR instrumentation contrasts with its exquisite sensitivity. Binding events and functional activity of nucleases (3) and proteases (4) can be monitored and quantified in real time based on observation of a single 20-nm nanoparticle — probably one of the world's smallest biosensing supports (5).