Purification of Monoclonal Antibodies from CHO Cell Culture Supernatants Using KANEKA KanCapA~(TM)

摘要

Developing robust and scalable purification processes for monoclonal antibodies (mAbs) can save time and money. KANEKA's protein A chromatography resin, KANEKA KanCapA~(TM), offers the required high-performance characteristics to develop platform purification processes for mAbs: high binding capacity, high alkaline stability, good elution profiles, low nonspecific binding, and robustness in column packing. Here we provide purification data of five different mAbs (two humanized IgGIs, two humanized lgG2s, and a chimeric IgGI) using KANEKA KanCapA~(TM). The Chinese hamster ovary (CHO) cell culture supernatants were kindly provided to us by our customer.