New Protein G Biosensor Brings 'Out-of-the-Box' Detection of Diverse Mammalian Antibodies

摘要

Today's antibodies are sourced from a diverse set of mammalian hosts whose isotypes have different affinities for protein A and protein G. ForteBio's new protein G biosensor provides an "out-of-the-box" solution for direct quantitation of the mammalian immunoglobulins that do not bind to protein A. Readings can be performed in buffer, chromatography eluents, conditioned medium, and cultured lysates using a convenient 96- or 384-well microplate format. Assay parameters can be adjusted to detect concentrations from 0.05 ug/mL to 2,000 ug/mL, and the biosensor can be regenerated using a simple protocol. Turn around 96 samples in 15 minutes, 384 samples in one hour. No plate washing, no microfluidics, no liquid waste.