Effect of Garcinia kola biflavonoid fractions on some biochemical parameters of P407-induced hyperlipidemic albino rats: a phytopreventive and phytotherapeutic studies

摘要

The phytopreventive and phytotherapeutic effects of Garcinia kola biflavonoid fractions on some biochemical parameters in Poloxamer 407 (P407)-induced hyperlipidemic rats were studied for a period of 21 days. Sixty mixed sex Wistar rats weighing 150-200 g were divided into two major groups: (1) phytopreventive group comprising the following subgroups: normal control, P407-induced hyperlipidemic control, and groups treated orally with atorvastatin as standard drug, root bark, stem bark, and seed biflavonoid fractions of G. kola, respectively, for 19 days and made hyperlipidemic with a single intraperitoneal injection on day 19; (2) phytotherapeutic group comprising similar subgroups that were induced by an intraperitoneal injection of P407 every 48 h and treated with atorvastatin, root bark, stem bark, and seed biflavonoid fractions for 21 days. In both groups, atorvastatin, P407, and the G. kola biflavonoid fractions were administered at 10, 500, and 200 mg/kg body weight, respectively. Blood samples were collected from the rats in all the groups at the end of the experiment for determination of serum levels of aspartate aminotransferase (AST), alanine amino-transferase (ALT), alkaline phosphatase (ALP), total protein (TP), albumin (ALB), total bilirubin (TB), conjugated biliru-bin (CB), and fasting blood glucose (FBG) using Randox kits. Serum concentration of AST was significantly (p<0.05) reduced by the root bark and stem bark biflavonoid fractions in phytopreventive and phytotherapeutic groups, respectively, compared to hyperlipidemic group. ALT levels of all induced rats were not significantly (p <0.05) changed when compared to normal rats. Analysis of liver function parameters showed all G. kola biflavonoid fractions prevented against further decrease in ALB levels in both studies and biflavonoid-treated subgroups had significantly (p<0.05) lower levels of TB in the phytopreventive group. TP, CB, and FBG levels of all induced rats were not significantly (p >0.05) different from the normal rats. These results highlight the efficacy of G kola (root bark, stem bark, and seed) biflavonoid fractions in the amelioration of some undesirable effects of hyperlipidemia.