Time Allowed for Dark Adaptation and the Parameters of the Fluorescence and Delayed Millisecond Luminescence Induction Curves: A Comparative Experimental and Theoretical Study

摘要

In in vivo experiments with Hibiscus rosa chinensis leaves, the fluorescence and delayed millisecond luminescence induction curves were recorded for various intervals of dark adaptation. The experimentally determined dependences of the parameter G = (F_m - F_s)/F_m on the dark time were compared with those obtained numerically using the theoretical model of photosynthesis described previously (Karavaev and Kukushkin, Biofizika, 1993, vol. 38, pp. 958-975; Kuznetsova and Kukushkin, 1st All-Russia Conf. Photobiologists, Pushchino, 1996, Abstracts p. 13). The G versus dark time plots, whether for induction of fluorescence or delayed luminescence, exhibited saturation. The fluorescence-to-luminescence saturation-time ratio was 1.40 as determined from the experimental and 1.46 from the simulated data. In both experiments and simulations, slow fluorescence induction curves were characterized by smaller G values than those for delayed luminescence. These results suggest that, compared with slow fluorescence induction, induction of delayed luminescence is a more sensitive index for assessing changes in the state of the photosynthetic apparatus.