The Polymorphism of Catechol-O-methyltransferase (COMT) and Hemochromatosis (HFE) Genes in the Radiocontaminated Regions Residents with Different Chromosome Aberration Frequency

摘要

The association between polymorphisms in genes COMT, HFE that takes part in oxidative stress regulation, and chromosome aberration frequency in lymphocytes was assessed in 278 female residents of radiation polluted regions of Central Russia: Bryansk (322 kBk/m(2)) and Tula Districts (Cs-137 - 171 kBk/m(2)). The C187G, G845A genotyping of HFE and G1947A (HA) of COMT was done by means of polymerase chain reaction-restriction fragment length polymorphism. Studied population was divided into 3 subgroups by level of chromosome aberrations per cell (0-2, 3-4, >5). There was shown statistically significant difference in distribution of COMT and HFE genotypes between the groups. The high frequency of chromosome aberrations (5%) was associated with homozygotes of the high activity COMT G/G and HFE CC. Heterozygotes for G1947A COMT and C187G HFE reveal negative association with the high frequency of chromosome aberrations and correspond to "resistance factors".