In biometric and biomedical applications, a special transporting mechanism must be designed for the μTAS (micro total analysis system) to move samples and reagents through the microchannels that connect the unit procedure components in the system. In view of this, an optimal fuzzy sliding-mode control (OFSMC) based on the 8051 microprocessor was designed and a complete microfluidic manipulated biochip system was implemented in this study, with a pneumatic pumping actuator, two feedback-signal photodiodes and flowmeters for better microfluidic management. This new technique successfully improved the efficiency of molecular biology reaction by increasing the velocity of the target nucleic acid molecules, which increased the effective collision into the probe molecules as the target molecules flow back and forth.
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