On independent denaturation of albumin and globulins' in the composition of human blood serum

摘要

The independent melting of albumin, #gamma#-globulin, transferrin, and protease inhibitors in the composition of donor blood serum was studied by differential scanning microcalorimetry. It was found that the number of domains in #gamma#-globulin in donor blood serum and in diluted solutions is the same, whereas the number of domains of albumin in solution and in the composition of blood serum is three and two, respectively. In blood serum, the N-terminal domain melts by the all-or-none mechanism. Therefore, the decomposition of peaks of the denaturation curve was under the assumption that the denaturation of blood serum proteins occurs by the all-or-none principle. It is assumed that comparing the calculated melting parameters (T_d, #DELTA#T_d, #DELTA#H_d, #DELTA#C_d) of domains of blood serum proteins of donor with the corresponding parameters of patients with oncological and nononcological diseases can be used as a basis for a more precise diagnostics of these diseases.