The impact of hybrid dysgenesis on the chromosome structure of Drosophila melanogaster ovarian nurse cells was studied. In the examined lines and interlinear hybrids (including those yielded by dysgenic crosses in the P-M and I-R systems of hybrid dysgenesis), disturbed chromosome synapsis was revealed. The disturbance was somewhat similar to that observed in interspecific hybrids. Quantitative analysis showed that the mean frequency of nuclei with defective chromosome pairing ranged from 60.4 to 76percent. FISH analysis of ovarian nurse chromosomes of Canton S X Berlin hybrids showed differences in the label localization in asynaptic homologs of arm 2L, which probably results in disrupted homolog pairing and reveal interlinear differences in localization of mobile genetic elements. Our results conform to Sved's model stating that hybrid dysgenesis is based on disorganization of the germline nuclear space.
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机译:研究了杂交发育不全对果蝇卵巢护士细胞染色体结构的影响。在检查的品系和线间杂种中(包括由杂种不成因的P-M和I-R系统中的不纯种杂交产生的杂种),揭示了染色体突触受干扰。干扰有点类似于种间杂种中观察到的干扰。定量分析显示,染色体配对缺陷的核的平均频率为60.4%至76%。对S X Berlin杂种的卵巢护士染色体进行的FISH分析表明,第2L臂突触同系物的标签定位存在差异,这可能导致同系物配对中断,并揭示了移动遗传元件的定位之间存在线性差异。我们的结果与Sved的模型相符,该模型指出杂种不成因是基于种系核空间的混乱。
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