ポリジメチルシ口キサン隔膜を介した水分ガス交換による微小流体細胞培養デバイスのオンチッブCO2インキュべーション

摘要

We demonstrated an on-chip CO2 incubation system suitable for microfluidic cell culture that doesnot need an external chamber or gas supply. A nested pair of reservoirs for liquids insulated by gas/vapor-permeable poly (dimethylsiloxane) (PDMS) enabled CO2 incubation of media stored in the inner reservoir. Thiswas achieved by only flowing heated sodium bicarbonate solution through the outer reservoir. The stabilizationcapability of temperature and pH was evaluated in situ with a miniature thermocouple and by absorptionspectroscopy of phenol red, respectively. The temperature and pH were stabilized within the range of 37.0 ± 0.2°Cand p117.3 ± 0.2 over at least 24 hours. This incubation capability was demonstrated through low-densitymicrofluidic culture of CV-1 epithelial cells under an inverted microscope for three days.