Immunosuppressant monitoring can be performed by UPLC-Tandem Mass Spectrometry in half the time needed for conventional HPLC-Tandem Mass Spectrometry, with comparable analytical performance.

摘要

Liquid chromatography-mass spectrometry (LC-MS) is an emerging technology for clinical laboratory measurement of small molecules, which promises superior analytical performance compared to immunoassay, with less reagent cost [1-6]. One limitation of LC-MS, however, is diminished throughput due to the inability to analyze samples in parallel , The rate-limiting step is chromatography, which requires approximately 3 min for measurements of sirolimus/tacrolimus and cyclosporine on the HPLC-MS/MS systems (Alliance 2795 HPLC-Quattro Micro MS/MS, Waters Corporation, Milford, MA) in the Brigham and Women's Hospital (BWH) Clinical Chemistry Laboratory. This limits throughput to ~ 120 samples/analyzer/shift, which is insufficient for a busy transplantation center without using multiple analyzers or performing the complex analyses on multiple shifts.