Macromolecular Sensing of RNAs by Exploiting Conformational Changes in Supramolecular Nanostructures

摘要

Here, we report on a ratiometric fluorescence biosensor based on self-assembled peptide nanostructures (SPN), which can respond to conformational changes induced by RNA ligand binding. The design of the SPN biosensor was inspired by the conformational stabilization and multimeriza-tibn behaviors of the HIV-1 Rev protein induced by cooperative protein-protein and protein—RNA interactions. Because conformation-sensitive SPN biosensors can orchestrate binding and signal transduction events, they can be developed as highly sophisticated and smart nanomaterials for biosensing.