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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Progestin and AdipoQ Receptor 7, Progesterone Membrane Receptor Component 1 (PGRMC1), and PGRMC2 and Their Role in Regulating Progesterone's Ability to Suppress Human Granulosa/Luteal Cells from Entering into the Cell Cycle
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Progestin and AdipoQ Receptor 7, Progesterone Membrane Receptor Component 1 (PGRMC1), and PGRMC2 and Their Role in Regulating Progesterone's Ability to Suppress Human Granulosa/Luteal Cells from Entering into the Cell Cycle

机译:孕激素和AdipoQ受体7,孕酮膜受体组分1(PGRMC1)和PGRMC2及其在调节孕激素抑制人颗粒细胞/黄体细胞进入细胞周期的能力中的作用

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摘要

The present studies were designed to determine the role of progesterone receptor membrane component 1 (PGRMC1), PGRMC2, progestin and adipoQ receptor 7 (PAQR7), and progesterone receptor (PGR) in mediating the antimitotic action of progesterone (P4) in human granulosa/luteal cells. For these studies granulosa/luteal cells of 10 women undergoing controlled ovarian hyperstimulation were isolated, maintained in culture, and depleted of PGRMC1, PGRMC2, PAQR7, or PGR by siRNA treatment. The rate of entry into the cell cycle was assessed using the FUCCI cell cycle sensor to determine the percentage of cells in the G(1)/S stage of the cell cycle. PGRMC1, PGRMC2, PAQR7, and PGR mRNA levels were assessed by real-time PCR and their interactions monitored by in situ proximity ligation assays (PLAs). These studies revealed that PGRMC1, PGRMC2, PAQR7, and PGR were expressed by granulosa/luteal cells from all patients, with PGRMC1 mRNA being most abundant, followed by PAQR7, PGRMC2, and PGR. However, their mRNA levels showed considerable patient variation. P4's ability to suppress entry into the cell cycle was dependent on PGRMC1, PGRMC2, and PAQR7 but not PGR. Moreover, PLAs indicated that PGRMC1, PGRMC2, and PAQR7 formed a complex within the cytoplasm. Based on these studies, it is proposed that these three P4 mediators form a complex within the cytoplasm that is required for P4's action. Moreover, P4's ability to regulate human follicle development may be dependent in part on the expression levels of each of these P4 mediators.
机译:本研究旨在确定孕酮受体膜成分1(PGRMC1),PGRMC2,孕激素和adipoQ受体7(PAQR7)和孕激素受体(PGR)在介导孕激素(P4)在人颗粒中的抗有丝分裂作用中的作用/黄体细胞。对于这些研究,分离了10名接受受控卵巢过度刺激的妇女的颗粒/黄体细胞,将其维持在培养物中,并通过siRNA处理去除了PGRMC1,PGRMC2,PAQR7或PGR。使用FUCCI细胞周期传感器评估进入细胞周期的速率,以确定处于细胞周期G(1)/ S阶段的细胞百分比。通过实时PCR评估PGRMC1,PGRMC2,PAQR7和PGR mRNA的水平,并通过原位邻近结扎分析(PLA)监控它们的相互作用。这些研究表明,所有患者的颗粒/黄体细胞均表达了PGRMC1,PGRMC2,PAQR7和PGR,其中PGRMC1 mRNA最丰富,其次是PAQR7,PGRMC2和PGR。但是,它们的mRNA水平显示出相当大的患者差异。 P4抑制进入细胞周期的能力取决于PGRMC1,PGRMC2和PAQR7,而不取决于PGR。而且,PLA指示PGRMC1,PGRMC2和PAQR7在细胞质内形成复合物。基于这些研究,建议这三种P4介体在细胞质内形成P4作用所需的复合物。此外,P4调节人卵泡发育的能力可能部分取决于这些P4介体中每一种的表达水平。

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