Colorimetric detection of WGA in carbohydrate-functionalized polydiacetylene Langmuir-Schaefer films

摘要

In this paper, carbohydrate-modified polydiacetylene (PDA) films prepared by Langmuir-Schaefer (LS) technique were used as biosensors for studying the interaction of wheat germ agglutinin (WGA) with the carbohydrate. Studies showed that the addition of WGA, specific to N-acetylglucosamine, induced the films to change their color from blue to red. UV-vis and Resonance Raman (RR) spectra confirmed the occurrence of molecular recognition. Surface pressure-area isotherms showed that glycolipid and polydiacetylene molecules had good miscibility on pure water subphase, which was significant for further applications as biosensor. (c) 2006 Elsevier B.V. All rights reserved.