Studies on development and application of high-throughput genomic and bioinformaticstools for citrus fruit physiology and breeding

摘要

Citrus is one of the most economically important fruit species in the world. The fruit is rich in the second metabolites for beneficial for human health, with diverse colors, fragrances and tastes. In addition, citrus is among the most difficult plants to improve through cross breeding approaches and to analyze physiologically varietal characteristics because of its the polyembryony, male sterility or self-incompatibility. Genome science technologies have advanced rapidly over the last decade, and have been adapted to address the challenges of the citrus breeding and physiological analysis. Expressed sequence tag (EST) analysis, DNA markers, linkage mapping and quantitative trait locus (QTL) analysis have promoted the efficient selection systems andanalysis of gene expression. Remarkably, three genome assemblies have been released to the public since 2011. Despite the challenges of working with citrus to understand the important characters of citrus, the result is insufficient. Expression analysisof many genes related to important characters and analysis of genome-wide genotyping among many varieties or the combination of these two analyses is necessary to understand important characters of citrus. This study was performed to provide the basis for comprehensive use of citrus genome information, which has been accumulated quickly.