DETERMINATION OF ACTIVE INGREDIENTS IN HAWTHORN AND HAWTHORN PIECE BY CAPILLARY ELECTROPHORESIS WITH ELECTROCHEMICAL DETECTION

摘要

Capillary zone electrophoresis with electrochemical detection has been used for the separation and determination of (-)-epicatechin, rutin, hyperin, chlorogenic acid and quercetin in hawthorn and hawthorn piece. Effects of several important factors including running buffer acidity, separation voltage and working electrode potential, were evaluated to acquire the optimum analysis conditions. The working electrode was a 300 цт carbon disc electrode at a working potential of +0.95 V (vs. SCE). Under the optimum conditions, the ana-lytes can be well separated within 16 min in a 75 cm length fused-silica capillary. The current response was linear over two orders of magnitude with detection limits (S/N = 3) ranging from 6.00 x 10~8 to 3.75 x x 10~7 g/mLfor all analytes. The method was successfully used in the analysis of hawthorn and hawthorn piece and the assay results were satisfactory.