首页> 外文期刊>Differentiation: The Journal of the International Society of Differentiation >Early endodermal expression of the Xenopus Endodermin gene is driven by regulatory sequences containing essential Sox protein-binding elements.
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Early endodermal expression of the Xenopus Endodermin gene is driven by regulatory sequences containing essential Sox protein-binding elements.

机译:Xenopus Endodermin基因的早期内胚层表达受包含必需的Sox蛋白结合元件的调控序列驱动。

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摘要

Abstract The Endodermin gene is expressed in the early endoderm and the Spemann organizer of Xenopus embryos. It has previously been shown to be a direct target of the early endodermal transcription factor Xsox17 (Clements et al., 2003, Mech Dev 120:337-348). Here we identify two adjacent control elements in the Endodermin promoter; these drive transcription of the gene in late-gastrula endoderm and contain consensus Sox-binding sites. We have analyzed one element in detail and show that it responds directly to Xsox17 and that the Sox sites are essential for endodermal expression in transgenic embryos. However, flanking regions on both sides are also essential, indicating that Xsox17 acts in concert with several DNA-binding partners.
机译:摘要Endodermin基因在非洲爪蟾胚胎的早期内胚层和Spemann组织者中表达。先前已证明它是早期内胚层转录因子Xsox17的直接靶标(Clements等,2003,Mech Dev 120:337-348)。在这里,我们确定Endodermin启动子中的两个相邻控制元件。它们驱动胃后胚层中基因的转录,并包含共有的Sox结合位点。我们已经详细分析了一种元素,并表明它直接对Xsox17作出反应,并且Sox位点对于转基因胚中的内胚层表达至关重要。但是,两侧的侧翼区域也是必不可少的,这表明Xsox17与几个DNA结合伴侣共同起作用。

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