Characterization of Bovine Interferon alpha(1): Expression in Yeast Pichia pastoris, Biological Activities, and Physicochemical Characteristics

摘要

A bovine interferon alpha (BoIFN alpha) gene that included signal sequence was amplified from bovine liver genomic DNA. The gene was named BoIFN-alpha(1) according to the position at which the encoded gene of the bovine IFN was located in the bovine genome. The sequence included a 23-amino-acid signal peptide and a 166-amino-acid mature peptide. The structural characteristics and phylogenetic relationships of the BoIFN-alpha(1) gene were analyzed. A recombinant mature BoIFN-alpha(1) (rBoIFN-alpha(1)) was expressed in the yeast Pichia pastoris. Physicochemical characteristics and antiviral activity were determined in vitro. Recombinant BoIFN-alpha(1) was found to be highly sensitive to trypsin and stable at pH 2.0 or 65 degrees C. It also exhibited antiviral activity, which was neutralized by a rabbit anti-rBoIFN alpha polyclonal antibody. This study revealed that rBoIFN-alpha(1) has the typical characteristics of IFN alpha and can be used for both research and industrial application.