Large scale production of a mammalian cell derived quadrivalent hepatitis C virus like particle vaccine

摘要

A method for the large-scale production of a quadrivalent mammalian cell derived hepatitis C virus like particles (HCV VLPs) is described. The HCV core El and E2 coding sequences of genotype 1a, 1b, 2a or 3a were co-expressed in Huh7 cell factories using a recombinant adenoviral expression system. The structural proteins self-assembled into VLPs that were purified from Huh7 cell lysates by iodixanol ultracentrifugation and Stirred cell ultrafiltration. Electron microscopy, revealed VLPs of the different genotypes that are morphologically similar. Our results show that it is possible to produce large quantities of individual HCV genotype VLPs with relative ease thus making this approach an alternative for the manufacture of a quadrivalent mammalian cell derived HCV VLP vaccine. (C) 2016 Published by Elsevier B.V.