Screening of mitochondrial proteins in winter rye, winter wheat, Elymus and maize with an immunochemical affinity to the stress protein 310 kD and their intramitochondrial localization in winter wheat.

摘要

An investigation of mitochondrial proteins with immunochemical affinity to winter rye (Secale cereale) CSP 310 was carried out using PAGE in members of the Graminacea with different tolerances to low temperature (maize (Zea mays), winter wheat (Triticum aestivum) and winter rye) and the low temperature tolerant wild grass, Elymus sibiricus. Western blotting showed that among the native mitochondrial proteins of all species investigated, there are proteins with immunochemical affinity to cytoplasmicCSP 310 with molecular weights 310, about 230 and about 140-110 kDa. Western blotting of submitochondrial fractions showed that in outer mitochondrial membrane there are polypeptides with molecular weights 60 and 58 kDa with immunochemical affinity to CSP 310 subunits. In the inner mitochondrial membrane, western blotting showed polypeptides with molecular weights 66, 60, 55 and 23 kDa. In the mitochondrial matrix, polypeptides with molecular weights 56 and 66 kDa were found.