EFFECT OF pH AND NAA ON IN VITRO RHIZOGENESIS IN.GYMNEMA SYL VESTRE R. Br

摘要

Gymnema sylvestre R.Br. is a woody climber (family Ascelpideaceae) having diverse medicinal uses. Various closely related components of gymnemic acid are used for diabetes in pharmaceutical industry all over the globe. A factorial randomized design experiment was conducted to study the effect of different pH regimes (3.4, 4.0, 4.4, 5.0, 5.4, 6.0 and 6.4), NAA (0, 3.75 fiM) and their all possible interactions on in vitro rooting (%) and root number per explant at 21 and 28 days after inoculation. A uniform B; basal medium was provided. The experiment laid out taking three replicates each of ten explants, i.e. microshoots with apical bud and two nodes. Various pH regimes and NAA at both stages of sampling and their interactions at 21 days after inoculation significantly influenced rooting (%) and root number per explant. The pH 4.4 enhanced rooting (%) by 168% and 100% at 21 days and 63% and 24% at 28 days in comparison to pH 3.4 and 4.0, respectively. Administration of 3.75fiMNAA significantly promoted root number per explant at both stages of sampling being 6.94 at 21 days and 5.28 at 28 days. Thus, administration of auxin at low pH facilitates induction and growth of in vitro rooting in Gymnema sylvestre.