A comparative study of the dietary lysine requirement of juvenile striped bass Morone saxatilis and sunshine bass M-chrysops x M-saxatilis

摘要

Previous reports have indicated there are significant differences in both the dietary lysine requirement and the metabolic rate of striped bass and its hybrids. However, there is very little directly comparative data to confirm these suggestions. A series of experiments was conducted to comparatively assess efficiency of protein and energy retention between striped bass Morone saxatilis and sunshine bass M. chrysops female x M. saxatilis male grown under identical culture conditions. In experiment one, a dose response study was conducted using digestible energy (DE) levels of 3,200 kcal/kg and 3,600 kcal/kg. At each level of DE, six levels of dietary lysine were fed to striped bass and sunshine bass. Ten sunshine bass weighing 3.48 +/- 0.08 g or six striped bass weighing 3.23 +/- 0.14 g were stocked into 15-L tanks supplied with singlepass flow-through fresh water. After 12 wk on the experimental regime, feed conversion ratios (FCR), weight gain, and % nitrogen (N) retention were determined. Nonlinear regression analysis indicated that the dietary lysine requirement of both bass groups was similar. The dietary lysine requirement was determined to be 2.0 +/- 0.08% of dry diet (or 6.0 +/- 0.26 g lysine/1,000 kcal DE), and 1.7 +/- 0.08% of dry diet (or 4.7 +/- 0.22 g lysine/1,000 kcal DE), for the 3,200 and 3,600 kcal DE/kg diets, respectively. A second experiment comparatively assessed growth, metabolism, and energy partitioning between striped bass and sunshine bass. Two diets were formulated to be isonitrogenous and isocaloric with a calculated DE level of 3,562 kcal/kg and contained lysine concentrations approximating the published dietary requirements of sunshine bass (low lysine = LL diet) and striped bass (high lysine = HL diet). The diets were fed at an average rate of 2% of body weight/d to 25 juvenile striped bass or hybrid bass held separately in eight 2000-L single-pass flow-through tanks supplied with freshwater. In general, growth performance of sunshine bass was superior to striped bass. Both bass groups performed better when fed the HL diet. Mean FCRs were 1.19 +/- 0.12 and 1.94 +/- 0.29, respectively, for hybrids and striped bass fed the LL diet (P = 0.066); and 1.17 +/- 0.07, and 1.58 +/- 0.08, respectively, for hybrids and striped bass fed the HL diet (P = 0.011). Mean % gain/d values were significantly higher (P = 0.001) for hybrids (2.77 +/- 0.85) than for striped bass (1.30 +/- 0.27) when fed the LL diet. When fed the HL diet, mean % gain/d was significantly higher (P = 0.003) for sunshine bass (2.72 +/- 0.83) compared to striped bass (1.51 +/- 0.25). Additionally, when fed the HL diet, sunshine bass had significantly higher percent nitrogen (P = 0.006) and energy retention (P = 0.014) when compared to striped bass. These results further document that sunshine bass are more efficient at protein and energy retention compared to striped bass in freshwater.