The use of dairy protocols for sperm cryopreservation of blue catfish Ictalurus furcatus

摘要

The commercial-scale production of fish by use of artificial (induced) spawning would require reliable, large-volume sources of sperm. Cryopreservation can be used to preserve and store sperm within commercial and research germplasm repositories, but is limited in its application to aquaculture. Straw volume and cooling chamber size restrict the quantity of sperm that can be frozen, and straws must be filled by hand. In contrast, the dairy industry has refined methods for freezing of bull sperm, including automation of straw filling and the use of large cooling chambers. These methods could be used for commercial-scale cryopreservation of fish sperm, although application would require testing. To supply sperm in large volumes, bags originally developed for swine semen could be cooled using dairy protocols and used as a container for fish sperm. The current study documented the use of commercial-scale dairy cryopreservation techniques for the production of hybrids of channel catfish Ictalurus punctatus (female) by blue catfish Ictalurus furcatus. Four cryoprotectants (methanol, dimethyl sulfoxide, dimethyl acetamide, and glycerol) were initially evaluated for use with blue catfish sperm. During May 2000 and March to April 2001, suspensions of blue catfish sperm were cryopreserved with 10% methanol in 0.5-mL French straws and in commercial swine semen bags (Cochette* bags, IMV International, Minneapolis, Minnesota, USA). Cryopreservation took place at a dairy breeding cooperative, using technology employed for bull semen. Sperm motility before freezing was 26 +/- 18% during Year 1 (2000) and 62 +/- 30% during 2001. Sperm were thawed at 40 C and used to fertilize the eggs of channel catfish (yielding hybrids). Motility after thawing for sperm frozen in 0.5-mL straws was 11 +/- 10% during 2000 and 50 +/- 24% during 2001. Motility after thawing was 41 +/- 17% for sperm frozen in swine semen bags in 5-mL aliquots and 43 +/- 10% for sperm frozen in 10-mL aliquots. Neurulation of eggs fertilized with thawed sperm from straws was 83 +/- 13% during 2000 and 54 +/- 27% during 2001. Neurulation was 57 +/- 24% using sperm frozen in swine semen bags in 5-mL aliquots and 55 +/- 10% using sperm frozen in 10-mL aliquots. There was no correlation between sperm motility before freezing (in 0.5-mL straws) and after thawing during 2000 (r = 0.52) or during 2001 (r = 0.49). In addition, there was no correlation between initial motility and neurulation of channel catfish eggs fertilized using thawed sperm during 2000 (r = 0.14) or during 2001 (r = 0.29). Sperm of blue catfish can thus be cryopreserved at a commercial scale using dairy protocols and can be made available for the production of hybrid catfish when viable eggs are available.