Use of diatoms to assess agricultural and coal mining impacts on streams and a multiassemblage case study

摘要

We developed and tested a species-level Bray-Curtis (BC) similarity to reference index and a genus-level diatom model affinity (DMA) index to quantify agricultural and acid mine drainage (AMD) impacts on streams in the Western Allegheny Plateau of southeastern Ohio. Decreased similarity to references sites was found in impaired streams, and diatom metrics further indicated how assemblages were impacted. Sites identified by index scores as impaired had significantly greater conductivity, PO4-P, Ca2+, Mg2+, Na+, Cl-, % pasture in the upstream watershed, and forest fragmentation than minimally impaired sites (p < 0.05). Percent acidophilic diatoms significantly increased with reduced alkalinity caused by mining impacts (p < 0.01). Relative abundances of species indicating low P and N status decreased with increased pasture and row crops in upstream watersheds (p < 0.01), whereas abundance of motile diatoms increased (p < 0.01). In a case study, diatom, macroinvertebrate, and fish assemblages were compared among 18 sites in a single watershed (6 sites along an AMD impact gradient, 6 NaOH-treated AMD sites, and 6 sites with no mining impacts). All assemblages indicated severe impairment by AMD, but fish were least useful because they were absent from 5 of the 6 sites. Macroinvertebrates indicated unimpaired conditions at treated sites, but fish signaled potential problems (lower than expected biomass and presence of deformities) despite high species richness and index of biotic integrity scores. Diatom indices indicated significant impairment at AMD and treated sites (p < 0.01). Diatom metrics and indices very effectively signaled agricultural and mining impacts at the regional scale and were especially useful in the case study because they provided finer resolution of AMD effects and additional information of ecological importance missed by or in conflict between macroinvertebrate and fish assemblages. The DMA and BC indices responded similarly to stressors at the regional scale, but BC more effectively signaled impacts at the watershed scale. However, DMA was an effective assessment tool that might be used more easily than BC by novice phycologists and watershed groups because it requires less taxonomic expertise. If implemented in future watershed or regional studies, diatom indices could greatly benefit policy, management plans, and current monitoring efforts.