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Imaging analysis of the in vivo bioreactor: a preliminary study.

机译:体内生物反应器的成像分析:初步研究。

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The in vivo bioreactor is a hermetically sealed, acellular hydroxyapatite scaffold coated with growth factors that has a pulsating vascular pedicle leash threaded through its center. Tissue-engineered bone is created in weeks while the bioreactor remains embedded under the skin of an animal. The bioreactor also provides a model to study osteogenesis and pathologic scenarios such as tumor progression and metastasis by creating a controlled microenvironment that makes skeletogenesis amenable to genetic and physical manipulation. Animal euthanasia is required to quantitate bioreactor osteogenesis through histomorphometry. Nondestructive measures of new bone growth within the bioreactor are critical to future applications and are the primary questions posed in this study. We compared microcomputed tomography and micro-MRI assessments of bioreactor osteogenesis with conventional histomorphometric measurements in 24 bioreactors and asked if new bone formation could be calculated while the animal was alive. Microcomputed tomography visually, but not numerically, differentiated engineered new bone on its coral scaffold. Dynamic contrast-enhanced micro-MRI demonstrated augmented vascular flow through the bioreactor. Three-dimensional imaging can nondestructively detect tissue-engineered osteogenesis within the implanted bioreactor in vivo, furthering the usefulness of this unique model system.
机译:体内生物反应器是一种涂有生长因子的密封无细胞羟基磷灰石支架,该支架具有穿过其中心的脉动血管蒂皮带。组织工程化的骨头可以在数周内产生,而生物反应器仍可以嵌入动物的皮肤下。该生物反应器还提供了一个模型,通过创建可控制的微环境使骨骼生成适合遗传和物理操作,从而研究成骨和病理情况,例如肿瘤进展和转移。需要动物安乐死以通过组织形态计量学定量生物反应器的成骨作用。生物反应器内新骨生长的非破坏性措施对于未来的应用至关重要,并且是本研究提出的主要问题。我们在24个生物反应器中将生物反应器成骨的微型计算机断层扫描和显微MRI评估与常规组织形态测量进行了比较,并询问在动物存活时是否可以计算出新的骨形成。微型计算机断层扫描在视觉上而非数值上,在其珊瑚支架上区分了工程化的新骨头。动态对比增强的显微MRI证实了通过生物反应器的血管流量增加。三维成像可以在体内无损检测植入的生物反应器内的组织工程性成骨,从而进一步增强了这一独特模型系统的实用性。

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