Occurrence of a Spontaneous Triploid Progeny from Crosses between Diploid Asparagus (Asparagus officinalis L.) Plants and Its Origin Determined by SSR Markers

摘要

The origin of a spontaneous triploid asparagus plant from crosses of 2x x 2x was investigated by SSR and flow cytometric analyses. One hundred and twenty-four progeny were obtained from crosses between a diploid female 'Gold Schatz' and a diploid male 'Hokkai 100'. SSR analysis proved that two and one genes were transmitted from the maternal and paternal parents, respectively, at each SSR locus of one progeny, 07M-61, whereas one gene each was from the female and male parents in the other diploid progeny. Triploidy of 07M-61 was confirmed by flow cytometric analysis. It was suggested that the triploid plant was derived from fertilization between an unreduced egg and reduced sperm nuclei, given its SSR genotypes. It was also suggested that the unreduced maternal gamete was derived from first division restitution (FDR) or second division restitution (SDR) with chiasma occurrence during meiosis. There were no noticeable morphological differences between the triploid and diploid progeny.