Effect of Agaricus blazei P-Glucan and Egg Shell Calcium Complex on Bone Metabolism in Ovariectomized Rats

摘要

This study was designed to evaluate the effect of Agaricus blazei ]3-glucan and egg shell calcium complex on bone metabolism in ovariectomized (OVX) rats. Forty Sprague-Dewley female rats, 10 weeks of age (248+-1.7 g), were divided into 4 groups and fed on the experimental diets for 6 weeks: sham operated control treated with normal diet containing 0.5% calcium (Sham-C), OVX-control treated with normal diet containing 0.5% calcium (OVX-C), OVX-bate-glucan group treated with bate-glucan diet containing 0.5% calcium (OVX-G), and OVX-bate-glucan egg shell calcium complex treated with OVX-P-glucan egg shell calcium complex containing 0.5% calcium (OVX-GE). Bone weight of femur was higher in the OVX-GE group than in the other OVX groups. Bone mineral density of femur was significantly different (p<0.05) among the experimental groups and showed the highest level in the OVX-GE group. Calcium absorption rate and retention were higher in the bate-glucan supplement groups than in the other groups (p<0.05). Alkaline phosphatase activities and osteocalcin levels of serum showed lower in the bate-glucan supplement groups than in the OVX-C group. Deoxypyridinoline crosslink values of urine, indicator of bone absorption, showed the lowest in the OVX-GE group. The bate glucan supplemented groups had a lower bone resorption ratio than in the OVX-C group. We concluded that bioavailability of calcium is higher inbate-glucan supplement groups compared to those in OVX rats. From the above results, these findings suggest the possibility of using bate-glucan egg shell calcium complex as a functional food material related to bone metabolism, even though there is no significant difference between the groups of bate-glucan and bate-glucan-egg shell calcium complex supplementation.