Physiological Activities of Hot Water Extract from Pine Bud (Pinus densiflora)

摘要

This research was performed to determine the antioxidant activity, nitrite scavenging activity and its inhibitory activity on angiotensin converting enzyme (ACE), xanthine oxidase, a-glucosidase, and elastase of hot water extract from pine bud (WPB).Antioxidant activity of WPB was measured by 2,2-diphenyl-l-pic-rylhydrazyl (DPPH) radical scavenging activity and superoxide dismutase (SOD)-like activity. DPPH radical scavenging activity and SOD-like activity of WPB were remarkably increased in a dose-dependent manner, and were about 71.4 and 85.4% at 2 mg/mL, respectively. The xanthine oxidase and ACE inhibitory activities were about 70.9 and 51.9% at 2 mg/mL of WPB, respectively. Nitrite scavenging activity of WPB was about 59.1, 53.8, and 39.5% onpH 1.2, 3.0, and 6.0 at 2 mg/mL, respectively. The WPB also showed elastase and a-glucosidase inhibitory effects. These results revealed that pine bud have strong antioxidant activity and positive effects on the inhibition of xanthine oxidase, ACE, and elastase.