Inhibitory Effects of Dicaffeoylquinic Acids from Artemisia dubia on Aldo-keto Reductase Family IBIO

摘要

The ethyl acetate fraction of Artemisia dubia showed a potent inhibitory activity on recombinant human AKRIBIO (rh AKRIBIO). Due to its potency, subsequent purification of ethyl acetate fraction led to seven compounds including five dicaffeoylquinic acid (DCQA) derivatives. Compounds were identified as 3,4-dihydroxybenzoic acid (1), quercetin 3-O-p-glucose (2), 3,4-DCQA (3), 3,5-DCQA (4), 4,5-DCQA (5), 3,5-DCQA methyl ester (6), and 4,5-DCQA methyl ester (7). A series of DCQA derivatives (3-7) showedinhibitory activity on rhAKRlBlO in the range of IC_(50)1.24-2.29 pM, whereas 3,4-dihydroxybenzoic acid and quercetin 3-6>-P-glucose showed no inhibitory activity on rhAKRlBlO.