Cloning of Tunicamycin Biosynthetic Gene Cluster from Streptomyces chartreusis NRRL 3882

摘要

The putative tunicamycin biosynthetic gene cluster was identified in Streptomyces clavuligerus with genome mining approach and this genetic information was used to isolate the tunicamycin biosynthetic gene set from S. chartreusis. Gene inactivation oftunl_(sch) abolished tunicamycin accumulation in S. chartreusis. confirming that the tunicamycin biosynthetic gene cluster was cloned.