In silico genomics analysis of weed Lantana camara and its proposed application in synthesis of polyhydroxybutyrate

摘要

Poly (3-hydroxyalkanoates) (PHAs) are a class of microbially produced polyesters that have potential applications as conventional plastics, specifically thermoplastic elastomers The most common PHA is poly-|3-hydroxybutyrate (PHB), and this polymer can accumulate up to 90% of the cellular dry weight of some bacteria. We report using Lantana camara, a weed for the production of PHB to provide a cheap and abundant source of this polymer. Maturase K gene (matK) of chloroplast is highly conserved in plant, which is involved in intron splicing. Based on a computational gene sequence analysis, we propose that the L Camara, a common weed, can be an inexpensive and abundant source for biological production of PHB. Our simulations focus on the maturase K (matK) gene -length of 1331 base pairs (bp) -of the choloroplasts, which affects the intron splicing. Our computational approach considers a reconstructed chloroplast genome of L Camara, the trnL-trnF intergenic spacer (IGS), and tmH-psbA IGS in the chloroplast genome. The Blastn result indicates that the Verbenaceae genus Lantana displays 86% sequence identity with that of Solanaceae genus Nicotiana. Phylogenetic tree was constructed to identify an ideal comparison of L camara matK gene and other plant species used for the insertion of phbA, phbB, and phbC enzymes, which regulate PHB synthesis. The former can be a good candidate for bioplastic biosynthesis in plant of family Verbanaceae. Our computational results propose that the chloroplast transformation may be useful in biological production of PHB.