Assessment of genetic fidelity of arecanut plantlets derived through direct somatic embryogenesis by RAPD markers.

摘要

Random amplified polymorphic DNA (RAPD) markers were used to evaluate clonal fidelity of plantlets derived through direct somatic embryogenesis from Yellow Leaf Disease (YLD) resistant arecanut palms. Pair wise genetic similarities were generated by Jaccard's coefficient using the RAPD banding pattern between each mother palm and its progenies (eight plantlets/palm). Mother palms and its progenies were showing high similarity (99% in one case and 98% in one palm). The low level of variability shown by plantlets of direct somatic embryogenesis can be exploited for large-scale multiplication of elite arecanut palms.