Comparison of enrichment vessels for recovery of Campylobacter spp. from broiler rinse samples.

摘要

The purpose of this study was to determine if the type of culture vessel used during incubation of an enrichment medium would affect the recovery of Campylobacter spp. from processed broiler rinse samples. Freshly processed broiler carcasses were rinsed, and the rinses were serially diluted. The diluted rinses were mixed with an equal volume of 2x Bolton enrichment broth (BEB; with or without blood) and placed in different container types for incubation and recovery of Campylobacter spp. The results showed that BEB in tissue culture flasks recovered Campylobacter from significantly more samples at higher dilutions than did BEB in resealable plastic bags (P<0.05), which was likely because of the gas permeability of the bags and greater media surface area in the flasks. The blood-free BEB in vented tissue culture flasks gave an equivalent rate of Campylobacter recovery as BEB for this sample type. The use of blood-free BEB and vented tissue culture flasks for the recovery of Campylobacter spp. from broiler rinse samples is suggested..