Hplc/dad analysis of phenolic compounds from lavender andits application to quality control

摘要

A reversed phase HPLC procedure is proposed for the deter- mination of eight phenolic compounds (2-0-glucosilcoumaric acid, o-coumaric acid, rosmarinic acid, apigenin- 7 -O-glucoside, coumarin, herniarin, luteolin, and apigenin) in lavender. The chromatographic separation was achieved using a reversed-ph~se Spherisorb ODS 2 (5 ~m particle size: 25.0 x 0.46 cm) column. From the several extractive solvents assayed, only ethanol was able to extract all the mentioned compounds. Best resolution was obtained using a gradient of water-formic acid ( 19: I) and ace- tonitrile. Ten samples were subjected to quantification, all of them showing a common composition pattern.